Where various areas of bright and dark spots represent the constructive (bright spots) and destructive (dark spots) regions. If this process were to be imaged, a speckle pattern would be observed, Therefore, given the large light intensity associated with DLS setups, many photons can interfere both constructively and destructively due to the many possible scattering angles. When the scattered waves interference is constructive, the DLS detector records high light intensity and equally, when waves interference is destructive, the detector records low light intensity.įigure: Constructive and Destructive Interference Waves, as is presented in the figure below. When two waves interact, they can combine in such a manner that the resultant wave is either an amplified or diminished superposition of the initially interacting The fundamental principle governing the determination of particle size by DLS is interference. We have selected cutting-edge direct imaging particle size analysers, such as Eyecon 2, to explain the fundamentals of image analysis which will give you an appreciation of some of the factors to consider in your choice of particleĭLS, sometimes referred to as Quasi-Elastic Light Scattering (QELS) and Photon Correlation Spectroscopy (PCS), is an optical method of determining particle size properties within a disperse sample by observing the change in scattered light intensity asĪ function of time. We have created a detailed review of industry-standard methods such as Dynamic Light Scattering (DLS) and image analysis, where we contrast the principles of DLSĪgainst image analysis. Exploring the particle size measurement landscape can be challenging and time-consuming.
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